Isolation and characterization of phosphate solubilizing bacteria from rhizosphere soil. After adaptation 1 gm of soil was inoculated in 100 ml liquid medium containing 1% glucose, 0. Isolation, screening and characterization of phosphate. The soil samples used for bacterial isolation were collected from rhizospheric soil fractions and rootfree soils from the salinity affected two. Isolation of phosphate solubilizing bacteria and their ability to solubilize calcium phosphate from the rhizosphere soils tomato, jasmine, onion, and maize, the total of 12 bacterial species of 32 samples were found enriched on pikovskayas agar plates. Aug 25, 2018 phosphate solubilizing bacteria psb can convert insoluble forms of phosphorus p to accessible forms. Pdf isolation of phosphate solubilizing bacteria and their. Pdf isolation of phosphate solubilizing bacteria from rhizospheric.
Phosphate solubilizing organisms play an important role in growth of the plants. Phosphate solubilizing bacteria psb function in soil phosphorus cycle, increasing the bioavailability of soil phosphorus for plants. Phosphate solubilizing bacteria psb are being used as biofertilizer since 1950s kudashev, 1956. Isolation and identification of potential phosphate. Isolation and screening of phosphate solubilizing bacterium from soil article pdf available in biotechnology. Phosphatesolubilizing fungi psf generally enhance available phosphorus p released from soil, which contributes to plants p requirement, especially in plimiting regions. Isolation and characterization of phosphatesolubilizing bacteria.
Phosphate solubilizing activities of all isolates were tested on national botanical. Phosphate solubilizing bacteria psb have the potential to enhance phosphate induced immobilization of metals to remediate contaminated soil. Homogenization of soil was carried out by keeping it on shaker for 30 minutes at 120 rpm at 27oc temperature. Jul 26, 2017 the soil samples were collected from the rhizosphere soil of chinese cabbage at a depth of 515 cm. Isolation of phosphate solubilizing microorganism psms.
Similar to our finding phosphate solubilizing bacteria were reported by many other workers from rice rhizosphere and also from the rhizospheric soil of other plants. Usually, one gram of fertile soil contains 101 to 1010 bacteria, and their live weight may exceed 2,000 kg ha1. Request pdf isolation and characterization of phosphatesolubilizing bacteria from rhizosphere soil different strains of phosphatesolubilizing bacteria psb were isolated from the. Experimental collection and preparation of soil samples. Experimental studies found that the strain pssg6 was effective in phosphate solubilization with phosphate solubilization efficiency index e 375 8. Xylose supplemented tris minimal rock phosphate medium. Rhizospheric bacteria were isolated from one gram of rhizosphere soil by serial dilution plating somasegaran and hoben, 1994 on pikovskayas agar plates containing tricalcium phosphate tcp 10 gl. Pdf isolation of phosphate solubilizing microorganism psms. Isolation of phosphate solubilizing bacteria from soil.
Isolation of phosphate solubilizing bacteria from maize. These include both aerobic and anaerobic strains, with a prevalence of aerobic strains in submerged soils. Isolation of the bacteria strains an aliquot of 100 l of the appropriate 101 to 105 dilutions of the soils samples were used to inoculate petri dishes containing nbrips phosphate growth medium of nautiyal, 1999, with tpr as sole source of phosphorus for the isolation of phosphate solubilizing bacteria. Tofazzal islama,b, abhinandan deoraa, yasuyuki hashidokoa, atiqur rahmana, toshiaki itoa, and satoshi taharaa a graduate school of agriculture, hokkaido university, kitaku, sapporo 0608589, japan b present address. Isolation identification and characterization of phosphate. However, there is a limit on the amount of phosphate which can be added to the environment due to the issue of eutrophication. Isolation of phosphate solubilizing bacteria and fungi. Potassium is a vital component of plant nutrition package limiting crop yield and. Homogenization of soil was carried out by keeping it on shaker for 30 minutes at. Isolation and characterization of ralstonia pickettii a.
Release of p by psb from insoluble and fixed adsorbed forms is an import aspect regarding p availability in soils. The present study was to extract the phosphate solubilizing bacteria from corn waste and its formulation in growth of pulses. Table 2 3 description of the source and the numbers of putative nitrogen fxing bacteria and phosphate solubilizing bacteria 50 table 2 4 enumeration of putativen fixing ang p solubilizing bacteria isolated from. Isolation and characterization of phosphate solubilizing bacteria from seagrass rhizosphere soil. Isolation of phosphatesolubilizing bacteria from tomato and potato samples approximately 2 g of soil was scraped from the roots of each sample and deposited into sterile tubes containing 2 ml of sterile deionized di water. Isolation and characterization of a phosphatesolubilizing. The rhizosphere soil was collected from all the 10 trees of one site and pooled together to form a sampling. Phosphate solubilizing bacteria psb have the potential to enhance phosphateinduced immobilization of metals to remediate contaminated soil. Moroccan journal of biology 20n 10 isolation and identification of potential phosphate solubilizing bacteria from the rhizosphere of lupinus hirsutus l. Isolation and phenotypic characterization of phosphate. Isolation and identification of phosphate solubilizing.
Pdf isolation of phosphate solubilizing microorganism. Isolation of phosphate solubilizing bacteria psb from maize rhizospheric soils. Isolation of efficient phosphate solubilizing bacteria. Microorganisms play an important role in the availability of soil phosphate to the root system and enhance the mobilization of phosphate in soil richardson, 2001. There are strong evidences that soil bacteria are capable of transforming soil p to the forms available to plant. The bio inoculants improve the shelf live and survivability of microbes in soil and stimulate the growth of the plants. Five bacterial strains with phosphatesolubilizing ability and other plant growth promoting traits. Phosphate solubilizing microbes have been reported for plant growth promoting and enhancing yield kapoor, mishra, and kuhkreja 1989. The analysis of phosphate solubilizing bacteria from soil sample was carried o ut in 22 o 32 17. Organic phosphate solubilization is also called mineralization of organic phosphorus, and it occurs in soil at the expense of plant and animal remains, which contain a large. Isolation and partial characterization of phosphate solubilizing bacteria isolated from soil and marine samples talat yasmeen mujahid 1, khaizran siddiqui 2, rifat ahmed 2. Isolation and characterization of phosphatesolubilizing bacteria from rhizosphere soil. After 47 likely in legume roots in which nodulation takes place. Phosphatesolubilizing bacteria psb function in soil phosphorus cycle, increasing the bioavailability of soil phosphorus for plants.
Isolation of phosphatesolubilizing bacteria and their ability to solubilize calcium phosphate from the rhizosphere soils tomato, jasmine, onion, and maize, the total of 12 bacterial species of 32 samples were found enriched on pikovskayas agar plates. This was placed in polythene bags and brought to the laboratory in ice boxes. Each test tube was vortexed thoroughly and a series of 10fold dilutions was prepared down to 109. More p can be released by inoculating soil with inorganicphosphatesolubilizing bacteria ipsbs. The serially dilution from 10 to 105 was achieved by trans ferring 1ml of mushroom residue solution from each preceding attenuation stage to the next. These isolates were identified using light microscopy and 16s rrna gene. To reduce the addition of mineral phosphorus to agricultural soils, research in naturally occurring phosphatesolubilizing microorganisms has been conducted for. Pdf isolation and screening of phosphate solubilizing. Tofazzal islama,b, abhinandan deoraa, yasuyuki hashidokoa, atiqur rahmana, toshiaki itoa, and satoshi taharaa a graduate school of agriculture, hokkaido university, kitaku, sapporo 0608589, japan. Phosphate solubilizing bacteria psb in hindi duration. Sep 01, 2016 isolation of bacterial colonies duration. Phosphate solubilizing bacteria with glucose dehydrogenase. Isolation and screening of potassium solubilizing microbes. The soil samples were collected from the rhizosphere soil of chinese cabbage at a depth of 515 cm.
Isolation of potential phosphate solubilizing bacteria. Rhizobacteria kurthia is isolated and characterized for phosphate solubilizing activity from the. Phosphorus solubilizing bacteria play role in phosphorus nutrition by enhancing its availability to plants through release from inorganic and organic soil p pools by solubilization and mineralization. A moderately halophilic bacterium was isolated from the sediment of daqiao saltern on the eastern coast of china, which also. Isolation of phosphate solubilizing bacteria to extract bacteria from soil, field moist soil was mixed with sterile 0. Psb were identified based on 16s rrna gene sequencing as pseudomonas synxantha, burkholderia gladioli, enterobacter hormaechei and serratia marcescens. In this study 50 collected soil samples were screened on the purpose of isolating psb. Isolation and characterization of a phosphorussolubilizing.
Isolation and characterization of phosphatesolubilizing. Pdf isolation and partial characterization of phosphate. In this study, isolation, screening and characterization of 35 isolates of phosphate solubilizing bacteria from different regions of karwar were carried out. Isolation and characterization of phosphatesolubilizing bacteria from seagrass rhizosphere soil. Isolation and identification of phosphate solubilizing and. Soil samples were collected from the maize rhizosphere soils of maize research station and college farm, college of agriculture, pjtsau, rajendranagar, hyderabad.
The production of organic acids by phosphate solubilizing bacteria has been well documented. Phosphorus solubilizing bacteria play role in phosphorus nutrition by enhancing its availability to plants through release from inorganic and organic soil p pools by. After nitrogen, phosphorus p is the second major essential macronutrient for plant development in soil, and the lack of p limits plant growth nautiyal, 1999. How to isolate phosphate solubilizing bacteria youtube. Isolation and application of salttolerant or halophilic psb will facilitate the development of salinealkali soilbased agriculture. Isolation of phosphate solubilizing microorganism psms from soil. Phosphate solubilizing bacteria psb can convert insoluble forms of phosphorus p to accessible forms.
Pdf isolation of phosphate solubilizing bacteria and. Phosphate solubilizing microorganisms are recognized as a solution to the. Isolation of phosphate solubilizing bacteria and their potential for lead immobilization in soil. Isolation of phosphate solubilizing bacteria from maize rhizosphere and their potential for rock phosphate solubilizationmineralization and plant growth promotion.
Dec 12, 2011 phosphatesolubilizing bacterial strains 6 nos. Nutritional requirement of every microorganisms may. Nutritional requirement of every microorganisms may get vary. Isolation and biological characterization of phosphate. Collection of plant samples and isolation of phosphate solubilizing bacteria chinese cabbage plants sampled from the research plots of the experimental field at cheongwon, chungbuk, republic of korea, were immediately transferred to the laboratory and processed within 24h. Identification and characterization of inorganicphosphate. Isolation of potential phosphate solubilizing bacteria from. Modi2 1department of biotechnology, kadi sarva vishwavidyalaya,gandhinagar 2life science department, school of sciences, gujarat university,ahmedabad author for correspondence abstract. A considerably higher concentration of phosphate solubilizing bacteria is commonly found in the rhizosphere in comparison with non rhizosphere soil. Isolation of phosphate solubilizing bacteria and fungi from. The collected soil samples were used for the isolation of phosphate solubilizing fungi on suitable culturing media. Hook and halodule pinifolia miki hartog in the vellar estuary.
The ability to solubilize fixed inorganic phosphorus p for plant growth is important for increasing crop yield. Phosphate solubilizing microorganisms as a phosphorus biofertilizer improve soil fertility by solubilizing insoluble phosphate salts and increase. A moderately halophilic bacterium was isolated from the sediment of daqiao saltern on the eastern coast of china. Isolation of phosphate solubilizing bacteria and their. We used 96well microplates instead of traditional 200mm petri dishes to rapidly screen ipsb strains for their solubilizing ability. Isolation of phosphate solubilizing bacteria and fungi from rhizospheres soil from banana plants and its effect on the growth of amaranthus cruentus l. Npkuniversity soil microbiology with harley smith duration. Lecturer5 department of microbiology malanakara catholic college mariagiri, kaliakkavilai 629053. Isolation and application of salttolerant or halophilic psb will facilitate the development of salinealkali soil based agriculture. For this purpose, 15 phosphorus solubilizing bacteria psb were isolated from wheat rhizospheric soils of peshawar and southern punjab region, pakistan. It precipitates in soil as orthophosphate or is absorbed by fe and al oxides through legend exchange. Phosphate solubilizing bacteria psb are soil bacteria, capable of turning insoluble form of phosphorus into the usable component for plants. Nagalakshmi department of microbiology, kanchi shri krishna college of arts and science, kilambi, kanchipuram631551, tn, india corresponding author abstract introduction phosphorus p is a major growthlimiting.
In this study, two psf, talajx04 and aspnjx16, were isolated from the rhizosphere soil of moso bamboo phyllostachys edulis widely distributed in pdeficient areas in china and identified as talaromyces aurantiacus. Three isolates selected between the 18 isolated strains according to their ability to hydrolyze. The effect of four phosphate solubilizing bacteria psb was studied on growth and aloina content of aloe barbadensis in soil containing tricalcium phosphate tcp. Isolation and identification of phosphate solubilizing and nitrogen fixing bacteria from soil in wamena biological garden, jayawijaya, papua suliasih, sri widawati microbiology division, research center of biology, indonesian institute of sciences bogor 16002. Isolation and characterization of phosphate solubilizing. Occurrence of phosphate solubilizing bacteria high proportion of psm is concentrated in the rhizosphere, and they are metabolically more active than from other sources.
Isolation and characterization of two phosphatesolubilizing. Five bacterial strains with phosphate solubilizing ability and other plant growth promoting traits. All the samples were analyzed for the presence of psb in laboratory by adopting standard procedure. Isolation of phosphate solubilizing microorganism psms from. Phosphate solubilizing microbes are found in soil mostisolation of phosphate solubilizing bacteria. Isolation and partial characterization of phosphate solubilizing bacteria isolated from soil and marine samples talat yasmeen mujahid 1, khaizran siddiqui 2, rifat ahmed 2, shahana. Isolation of phosphate solubilizing bacteria from white lupin. In this study, two psf, talajx04 and aspnjx16, were isolated from the rhizosphere soil of moso bamboo phyllostachys edulis widely distributed in pdeficient areas in china and identified as.
Isolation and characterization of phosphate solubilizing microbes from agricultural soil t. Isolation, characterization and identification of potassium solubilizing bacteria from rhizosphere soil of maize zea mays. These isolates were identified using light microscopy. Phosphate solubilization, rhizospheric soil, physiological studies.
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